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KMID : 0358219960230030351
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Korean Journal of Fertility and Sterility
1996 Volume.23 No. 3 p.351 ~ p.356
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Effect of Nitroprusside on the Sperm Motility, Viability, and Reactive Species Generation
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민부기
이희민/김기석/이희섭/김홍곤/홍기연/이봉주
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Abstract
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Objective: @EN To analyze the direct effect of nitrc oxide, generated from sodium nitroprusside, on sperm motility and reactive oxygen species.
@ES Design: @EN Human sperm samples were treated to allow swim-up and washing. And the samples were devided into four aliquots. Each aliquot was incubated with either concentration at 0, 100nM, 10μM, 1mM of nitroprusside.
@ES Intervention: @EN Samples were measured chemiluminosence for reactive oxygen species of each aliquot with concentrations at 0, 100nM, 10μm, 1uM of nitroprusside at allowing swim-up and washing of sperm.
@ES Man Outcome Measures: @EN Percent motion parameters and viability were asse-ssed at 0, 3, 6, 12, 24 hours incubation.
@ES Results: @EN the percent viablity was lower slightly in control group (50.2%) than that in sperm treated with 100nM of nitroprusside (57.5%) at 24 hours after incubation, while was reduced significantly in sperm with concentra-tion of 10μM
(42.1%)
and 1mM (21.3%) of nitroprusside at 6 hours after incubation. And the sperm treated with 1mM of nitroprusside was immotile totally at 6 hours after incubation. The straight line (35.3±5.6%), the rapid forward (37.2±6.4%) and the weak
curvilinear
velocity (9.6±2.4%) were more favorable comparing with those (32.4±4.2%, 30.0±7.8% and 18.0±4.6% respectively) in control group at 3 hours after incubation, but reduced significantly in sperm treated with 10μM and 1mM of nitroprusside. The
levels
of reactive oxygen species in control (700 c.p.m.) is lower significantly than that in each experimental groups of sperm treated with nitroprusside. And the levels of reactive oxygen species were 2200 c.p.m. in 100nM, 6200c.p.m. in 1μM
respectively.
@ES Conclusion: @EN These results suggested that the concentration of 100nM of nitroprusside on sperm is beneficial to the maintanance of viablity and motile velocity, but detriment in high concentration of 10μM or 1mM of nitroprusside.
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KEYWORD
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